The objective of this study was to evaluate the development of ewe follicles and oocytes viability following an intrauterine transplantation of the ovaries into pseudopregnant rabbit. The experiment was conducted in day 1 of pseudopregnant rabbit. Following transplantation for 5, 7, and 9 days, the ovaries were recollected. The development of follicles determined by counting the number of follicles in paraffin embedded ovaries after staining with haematoxylin-eosin (HE). The viability of oocytes was determined by slicing the ovaries. Oocytes were incubated in CO2 incubator with 5% CO2,38°C for 24 hours. After maturation, the oocytes were stained with 2% aceto-orcein to determine the nuclear oocytes status. The result showed that follicles were detected in all stages of their development (primordial, primary, preantral, and antral follicle stages), but their number decreased significantly (P<0.05) 5, 7 or 9 days after transplantation, except for those at primordial stage which at day 5 post-transplantation (634.7±56.88) were not significantly different to the control (683.7±61.55). After maturation, the oocytes that were able to reach the M-II phase at day 5 and day 7 post-transplantation were 35.05% and 35.24% respectively. They were significantly (P<0.05)>
Key word: ovarium, follicles, oocytes, viability, post-intrauterine transplantation
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